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The Prime Script RT Reagent Kit (Takara, Japan) was used to synthesize complementary DNA (cDNA), and qRT-PCR was performed using the SYBR Green Premix Ex Taq (Takara, Japan) on the LightCycler480 instrument (Roche) according to standard protocols

A number of studies in the 1990s confirmed dysregulation of PKC isoforms in symptomatic bipolar disorder, with the most consistent finding being an increase in PKC activity associated with manic symptoms

PMID 31547791

To this end, we performed a post hoc analysis on a subset of the study population that is characterized by increased oxidative stress, as determined by the values above the median for MDA, while showing low levels of baseline glutathione levels, as characterized by values below the median