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Patients with high NNMT levels tend to have poor prognostic parameters and overall survival (OS) and disease-free survival (DFS) (27, 45)

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for 6 min, following which the reaction was terminated by the addition of a mixture of 10% trichloroacetic acid and 4% methanol, vortexing for 5 s, and centrifuging at 14,000 rpm for 2 min to precipitate the protein