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Isolated RNA was purified using DNase I (Sigma) before conversion to cDNA using the GoScript Reverse Transcription system (Promega)

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SAT and OASTL catalyze the last steps of cysteine biosynthesis, whereas GSH1 is the rate-limiting enzyme of GSH biosynthesis, and PCS is involved in phytochelatin biosynthesis ( 1 hybrids (CEMK-9 X Xanthi, TRI-2 X Xanthi, CEMK-9 X NahG and TRI-2 X NahG)

The crystallite size was calculated using the following equation: where D is the average crystallite size (nm), K is the shape factor (~ 0.9), is the X-ray wavelength (1.5406), is the full width at half maximum (FWHM) of the diffraction peak expressed in radians, and is the Bragg diffraction angle