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For transwell experiments, inserts were placed in 50 mL centrifuge tubes containing 10 mL 1 PBS (pH 7.4), and vortexed to separate the fungal cells from the membrane

Recent clinical trials on glutamine metabolism inhibition mainly focuses on using CB-839 to target glutaminase (NCT02071862, NCT02071888 and NCT02071927 for solid tumors, lymphoid, and myeloid malignancies respectively) [24, 62]

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