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Real-time quantitative PCR analysis Using the RNAiso Plus kit, total RNA was extracted from gill tissue samples, and cDNA was produced via reverse transcription with the Prime Script RT kit

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Moreover, there is a growing consensus that a healthy microbiome might not be defined as an idealised assembly of specific microbe populations, but that the microbes community should be able to perform a set of metabolic functions together with its host, although this set of metabolic functions is still to be defined

J., Dumas, E