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Although this approach allowed precise manipulation of temperature as well as detailed assessment of migratory behavior, metabolic activity, and transcriptomic remodeling, it does not fully recapitulate the complex thermal gradients and microenvironmental cues encountered by T cells in vivo

For gene expression analysis, 1 g RNA was used for cDNA synthesis using a qScript cDNA Synthesis kit (Quanta Bio, 66196756)

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