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Tissue GST activity assay: Enzymatic reaction: Homogenate supernatant (ml) Mix sufficiently, centrifugate 3500~4000rpm for 10 minutes, take supernatant for chromogenic reaction Chromogenic reaction: Calculation: Unit definition: 1mg tissue protein reacts at 37 for 1 minute, subtract non-enzymatic reaction, 1mmol/L GSH concentration reducing in reaction system is considered as 1 enzyme activity unit (U)

Bateman ED, Hurd SS, Barnes PJ, Bousquet J, Drazen JM, et al: Global strategy for asthma management and prevention: GINA executive summary

Immunostaining results showed an increase in Ki67 expression in the G/D-CuP group compared to other groups, while the control group exhibited minimal positive cells on day 7 (Fig