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2.2 Primary culture of myocytes and thermal stress trial 2.2.1 Primary culture of myocytes Juvenile fish, each weighing approximately 5 g, were dissected under sterile conditions to obtain muscle tissue, which was then cut into pieces and transferred to a 24-well cell culture plate

These posttranslational modifications may also be a prerequisite for interactions of BDNF with chaperones or sorting receptors that guide the secretory protein BDNF predominantly to vesicles of the regulated secretory pathway (reviewed in Lemann and Brigadski 2009)

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