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Further, high concentrations of L-DOPA in the mutant supernatant had potent radical scavenging activity and fully quenched the ABTS chromophore monocation an effect that was comparable to the antioxidants GSH, cysteine, ascorbic acid, and the GSH precursor -Glu-Cys (Fig

Nowadays, metabolomics is recognized as a powerful tool for assessing the positive and negative effects of drugs under different exposure conditions ( Furthermore, the combination of metabolomics with in vitro models represents a great step forward in the use of human-relevant non-animal alternatives in toxicology

Step 5: Analytical Quantification (HPLC/UV/Fluorescence) The concentration of the drug in the free and/or encapsulated fractions is quantified using the chosen analytical technique

Moreover, the contribution of presynaptic EAAT2 to the glutamate uptake has been acknowledged in hippocampal neurons (Furness et al., 2008)