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During single mitochondrial imaging experiments, single excitation HyPer7 intensities per mitochondrion were normalized to that same mitochondrions area in each frame, which similarly limited expression-based confounds

At the end, sections were cover-slipped for microscopic inspection after being dehydrated, cleared in xylene, and reverse-stained with hematoxylin

This review explores the osmoregulatory responses of teleost fish to salinity changes, as well as the roles of gut microbiota, metabolomics, and transcriptomics in these responses

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