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Required Equipment and Materials Plate reader (405 or 415 nm filter) 96-well microplate Incubator (37C) 20 l and 200 l pipettes, a multi channel pipette 5-Sulfosalicylic Acid (SSA) Solution Ethanol Assay Procedure Sulfasalazine Alters Intracellular Metabolites and Increases ROS in A549 Cells Induction of Ferroptosis by Erastin Erastin is a known inducer of ferroptosis

Lysates were injected onto the column and chromatography was performed on the AKTA-FPLC system (GE HealthCare) with CHAPS buffer

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Neural Tube Defects (NTDs): folic acid deficiency can prevent the neural tubes to close around spine and brain in early stage of pregnancy