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100 L reactions were performed at 30 C for 3 h, in 50 mM sodium phosphate buffer (pH 8.0) containing 0.2 mM FeSO 4 , 2 mM KG, 2 mM ascorbate, 1 mM of substrate 12 or 13 , and 10 M of Cp1A

There are various potential causes of vitamin B12 deficiency besides pernicious anemia, which gastrointestinal surgery long-term use of certain medications, such as proton pump inhibitors genetic intrinsic factor defects dietary deficiency Pernicious anemia requires lifelong treatment

The commenter stated that CMS's own Information Collection Review document acknowledges the breadth of the undertaking, but fails to account for the extensive coordination needed with vendors and suppliers, from whom much of the requested data must be sourced