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Nuclei were stained using 0.1 ug/ml DAPI (Thermo Fisher Scientific) for 10 minutes at room temperature, followed by PBS washing and mounting using Fluoromount-G (SouthernBiotech, Birmingham, AL, USA)

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Li et al., 2008), it is difficult to only specifically target the spread of -syn, and so multiple approaches rely on targeting -syn that is associated with its spread, while also partially targeting endogenous -syn

- Improved Muscle Definition: Supports the reduction of body fat while preserving lean muscle mass