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Analysis of the ER redox state with ERroGFP S4 Fluorescence images of HeLa cells stably expressing ERroGFP S4 were acquired by an LSM 510 META confocal microscope (Carl Zeiss, Germany) or an FV3000 confocal laser scanning microscope (Olympus, Tokyo, Japan) and quantified fluorescence intensity ratio (Ex405/Ex458 for the Carl Zeiss lsm) or (Ex405/Ex445 for the Olympus lsm) by using Image J as described previously 19