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After a 1-day incubation, the cells were observed under a microscope to confirm that they had grown to cover the entire bottom of the insert

Breastfeeding Breastfed infants of mothers receiving biologics, immunosuppressants or combination therapies have a similar risk of infection and development milestone at 12 months to non-breastfed infants and infants unexposed to these medicines[117]

(Halliwell and Gutteridge, 1985)

Peptidase assays Stocks of each reagent were prepared at 4 final concentrations, LLVYAMC (400 M), hs 26S proteasome stock (400 nM), FAT10 (60 M) and NUB1 (60 M), in reaction buffer supplemented with 5 mM ATP