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A systematic review and dose-dependent meta-analysis of the intake or blood concentrations of dietary -carotene, lycopene, and -carotene were also conducted to evaluate the risk of prostate cancer (Wang et al

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After extensive washing, samples were eluted with lysis buffer supplemented with 500 mM imidazole and loaded onto a HiLoad 26/600 Superdex 75 pg column (GE Healthcare) equilibrated in 25 mM sodium phosphate pH 6.7 and 150 mM NaCl