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At the same time, compared to HCs, the levels of a wide range of metabolites such as glutamine, asparagine, N-Formimino-glutamate, putrescine, serotonin, kynurenine, 2-Aminomuconate, Cys-Gly, 5-Aminolevulinate, and pyridoxine were reduced in the plasma of R group ( Figure 5C , Supplementary Figure 4A )

(C) ROS level in basal condition (5% FBS) and serum-deprived HMVECs treated with PBS (vehicle, 12h), L-aminocarnitine (L-amino, 1M, 12h) and/or PLC (1mM, 12h)

Second, in the 1980s, vascular endothelial cells were shown to produce nitric oxide from l-arginine, which explained the biological effects attributed to endothelium-derived relaxing factor (Furchgott and Zawadzki 1980