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doi: 10.3390/medicina57121303 175

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10.1186/s12916-023-02874-y 73

Western blotting assay After KLE and Ishikawa cells were treated with 0.01, 0.1, and 1 mg/mL ASP extracts for 24 h, the cells were lysed in RIPA buffer supplemented with protease inhibitors (Roche, Seattle, WA)