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Total RNA was isolated using a freeze-thaw-vortex method, which involved three cycles of alternating liquid nitrogen freezing, room temperature thawing, and 30-s vortexing

National Research Council, and Committee on Diet and Health

In order to assess whether GSTP1 was able to interact with E7 oncoproteins from high-risk HPVs other than HPV-16, we challenged recombinant N-6His-GSTP1 with radiolabeled IVT HPV-33 and HPV-18 E7 molecules, which interacted in vitro specifically with recombinant GSTP1, but not with recombinant RCC1, which was used as a control (Figure 1G)

For example, the fatty acids are derivated from coconut or palm kernel oil