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Effect of increasing the ionic strength and diluting the cell lysate in the presence and absence of BugBuster without sonication Induced cells were next used in triplicates and re-suspended independently in LSB-2 containing 75, 150 or 300 mM NaCl keeping all the other components (10 mM Tris, 1 mM EDTA, 10 mM -mercaptoethanol and 1 mM PMSF) intact, followed by incubation with BugBuster and processed without any sonication

Mammalian target of rapamycin (mTOR) can be an important target of the non-genomic TG pathway

doi: 10.1038/sj.jcbfm.9600312 167 Sanz-MorelloB.AhmadiH.VohraR.SaruhanianS.FreudeK

[PMID: 3662815] 5