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J Infect Dis (2002) 185:111520.10.1086/340042 6 SzakmanyTHauserBRadermacherP

Scaffolds were incubated with primary antibodies overnight at 4 C and with secondary antibodies for 2 h at room temperature

Regulation of preproinsulin mRNA stability in health and diabetes Preproinsulin mRNA has a long half-life, which is mainly regulated by a conserved polypyrimidine tract and a UUGAA-motif in its 3-UTR [13, 16]

To clarify whether the first N-terminal residues of BNIP3 represent the discrete structural unit that directly can activate BAX, we conducted an established in vitro BAX activity assay utilising BNIP3-peptide 1-49, other BH3-only BAX activators (BIM and PUMA) as positive controls 38 , and bovine serum albumin and the antiapoptotic BCL XL as negative controls 2