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Description

Allosteric modulation of Drp1 mechanoenzyme assembly and mitochondrial fission by the variable domain

doi:10.1001/jamaoto.2016.1700

After sample denaturation and electrophoresis, membranes were blocked with 5% milk in TBS-T (120 mM Tris-HCl, pH 7.4, 150 mM NaCl, and 0.05% Tween 20) for 1 h at room temperature