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Often there are associations and correlations found but its rare and extremely exciting to find something that actual a direct effect

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Indeed, we could assemble octasomes with both (H3/H4) 2 and (CENP-A/H4) 2 tetramers on 601 positioning and on natural centromeric alpha-satellite DNA, albeit 2x(CENP-A/H4) 2 octasomes consistently assemble with lower efficiency (Fig S1B)

When combining approaches, timing considerations apply