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Cells were maintained in collagen-coated flasks in Rat Skeletal Muscle Cell Growth Medium (Cell Applications, Inc, San Diego, CA, USA) and myoblasts at passage 3 were used for experiments

Multiple randomised, placebo-controlled trials have demonstrated significant symptom improvements: DAO supplementation is most effective when used alongside dietary modification rather than as a standalone substitute

Second, both PRF and MeCbl were applied concurrently, thus it is uncertain whether the observed durable pain relief was driven by PRF alone, Vitamin B12 alone, or a synergistic combination

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