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An equal volume of dye Mastermix (1 M JC-1, 0.005% digitonin, 10 g ul 1 oligomycin, 5 mM -mercaptoethanol in DTEP buffer) was added, and after 10 min at room temperature, the cells were added on top of the peptide template at a concentration of 40,000 cells per well

In silico study Docking and molecular dynamic simulation study The docking and MD simulation were used to check the interaction between receptor and ligand, as described by Pharmacokinetic measurement The pharmacokinetic (ADME) characteristics of the substances have been evaluated using the SwissADME online approach

19 GoswamiS.AsraniP.Ansheef AliT

Within the glow blend, GHK-Cu comprises the largest component by mass, reflecting its higher dosing requirements relative to the other peptides