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The unrestricted pathway, due to epithelial cell damage, is independent of TJs and allows for the pathologic flux of luminal content across the epithelium

Some practitioners suggest cycling, with a 6-12 week usage period followed by a 4-8 week break between cycles

Science of the Total Environment, 801: 149709 Mandal A, Sinha C, Jena A K, Ghosh S, Samanta A (2010)

Thus, dissecting the contributions of E1, E2, and E3 components in cardiomyocytes is fundamental to understanding how UPS perturbations drive cardiovascular diseases