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2C that tryptophan fluorescence spectra has been red shifted followed by large reduction in fluorescence intensity at max indicating certain chromophore(s) that was buried in the core of the protein have been shifted to the polar environment

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For in vivo experiments, high SNR and DTOF resolution of our experimental setup with SNSPD allowed us to use all capabilities of the TD-DCS technique and not only extract gated BFI but also derive optical properties for each subject individually which then were used in the fitting algorithm unlike similar works 19,46 on TD-DCS-SNSPD systems using fixed optical properties for all subjects

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