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Cells were stained with 0.2 M MitoSOX in prewarmed hanks balanced salt solution (HBSS), incubated in the dark at 37C and immediately analyzed using a FACS Canto II flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA)

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FIGURE 1 2.1 NO synthase isoforms and their roles in CRC Each isoform plays a unique role in the pathophysiology of CRC, as mentioned in Table 1