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In this regard, MAD fundamentally contrasts with the amyloid cascade hypothesis in the notion that A is the central driver of AD, but incorporates it in a more holistic perspective with microbes instead as the central driver

Remove excess free radicals that cause abnormal functioning of brains and organs

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The cells were resuspended in lysis buffer (50 mM HEPES-NaOH pH 8.0, 300 mM NaCl, 10% glycerol, 1 mM MgCl 2 , 5 mM -mercaptoethanol 4 g/ml Avidin, 500 U Pierce Universal nuclease for cell lysis, 1 mM AeBSF, 1 Roche cOmplete EDTA-free protease inhibitor tablet per 100 mL of lysis buffer), lysed by sonication and clarified by centrifugation at ~ 35,000 x g for 45 min