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Cells were then resuspended in 500 L of free HBSS, strained through a 40 m cell strainer (BD Falcon), and analyzed using a flow cytometer (BD LSRFortessa Cell Analyzer, BD Biosciences) equipped with 488 nm laser for excitation

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Hosie MJ, Addie D, Belk S, Boucraut-Baralon C, Egberink H, Frymus T, Gruffydd-Jones T, Hartmann K, Lloret A, Lutz H, et al

The released GH acts on the liver and other tissues to produce IGF-1, the insulin-like growth factor that mediates most of GH's anabolic and repair effects