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An initial gradient of methanol: dichloromethane (0-25% over 10 min) was used, followed by a second gradient of chloroform:methanol:water (35:14:1, isocratic over 15 min)

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11 The F.D.A

Interestingly, TQ shows a concentration-dependent behavior, it acts as an antioxidant at low doses while exhibiting pro-oxidant effects at higher concentrations [14]