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Extracts of the medium were incubated at 80 C for at least 15 min before being centrifuged at 15,000 g for 20 min

The tagged proteins are then passed through a column of beads containing covalently attached, immobilized nickel(II) (Ni 2 + .) This His-tag binds tightly to the immobilized metal ions because the side chain of Histidine, imidazole, has a specific binding affinity to metal ions (in this case, nickel II)

J Transl Med 16:12 Adeyemi DH, Obembe OO, Hamed MA, Akhigbe RE (2024) Sodium acetate ameliorates doxorubicin-induced cardiac injury via upregulation of Nrf2/HO-1 signaling and downregulation of NFkB-mediated apoptotic signaling in Wistar rats

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