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The Ct values were obtained from Ct values of interest genes subtracting that of loading control

We found that the FB1 treatment increased the expression of all GST genes in both wheat genotypes in a genotype-dependent manner compared to untreated controls

Selenium requirements as discussed in the 1996 joint FAO/IAEA/WHO expert consultation on trace elements in human nutrition

In vivo ubiquitylation assay HEK293 cells were co-transfected with the indicated plasmids or siRNA oligos for 48 h, and then treated with relevant activators or inhibitors