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The generated LC-MS raw data were processed with Spectronaut (version 18.5.231110) (Biognosys) using the standard settings of the directDIA workflow, except that quantification was performed on MS1, with a human SwissProt database (www.uniprot.org, 20422 entries)

With regard to the remaining four spots, spots 4 and 10 were identified as SH3 domain containing RING finger protein 3 and T complex protein 1 subunit (TCP1), respectively, with isoelectric points of 9.99 and 7.67 (Table I)

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The most important consideration is the thermodynamic aspect, which determines whether the reaction is favorable or unfavorable