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Figure 10 7 Limitations and future directions of ferroptosis in neurodegenerative diseases Although mounting evidence highlights the critical role of ferroptosis in the onset and progression of neurodegenerative diseases, and its status as a novel mode of cell death is increasingly recognized, the field remains at an early stage of conceptual integration and mechanistic exploration

Further, the cells were washed twice with ice cold Dulbeccos phosphate-buffered saline (DPBS) and fixed with 4% formaldehyde for 20 min and permeablized in 0.1% Triton X-100 for 10 min, washed, and mounted using Fluoromount (Sigma) for imaging

Water level (the height of the lake surface above sea level) is influenced by many factors, including precipitation, snowmelt runoff, drought, evaporation rates, and people withdrawing water for multiple uses

Reference Silva, Martins, Silva and Martins2014)