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Borneol exerts its antipruritic effects by inhibiting TRPA1 and activating TRPM8

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To do this, rats were sacrificed and the posterior gastrocnemius muscles on the injured and uninjured hind limbs were weighed and the wet weight-loss of gastrocnemius muscles was calculated by the following equation: 2.9.4 Functional assessment of sensory recovery (Hot plate latency test) Twelve weeks post-surgery, the rats were evaluated for hot plate latency (HPL) by placing their injured limbs on the center of a hot plate (56 C) and recording the time until they reacted (by jumping or licking their paws)

Prior to imaging, seedlings were rinsed in sterile water and stained with 500 nM propidium iodide (PI, 50 M stock solution in H 2 O) for 1 minute, then rinsed again with water to get rid of the excess staining