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The angiogenic effect of copper has been well-recognized, which is presumed to attribute to the regulation of factors involved in vessel formation and maturation, especially the pro-angiogenic factor, namely vascular endothelial growth factor (VEGF), and its major transcription factor, namely hypoxia-inducible factor-1-alpha (HIF1) [36]

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[9] Chemistry methods were widely-used, including oxygen radical absorbance capacity (ORAC) [10] , total radical-trapping antioxidant parameter (TRAP) [11] , Trolox equivalent antioxidant capacity (TEAC) [12] , total oxyradical scavenge capacity (TOSC) [13] , the peroxyl scavenging capacity (PSC) [14] , the ferric reducing/antioxidant power assay (FRAP) [15] , and the DPPH free radical method [16]