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Choline-deficient HFD rat Six- to 8-week-old male Wistar rats (Charles River Laboratories) were fed an L-amino acid rodent diet with 60 kcal% fat with no added choline and 0.1% methionine (Research Diets, A06071302) for a total of 12 weeks

Pharmaceutical Chemistry & Structural Characteristics From a pharmaceutical chemistry perspective, the L-glutamine structure consists of an amide side chain attached to a five-carbon amino acid backbone, contributing to its nitrogen-donating capacity

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However, whether interactions among these components contribute to the enhanced effects of WEH remains to be investigated