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To infect MEFs, 500 l of virus-containing supernatant (approximately 40% of the total) was combined with 1 ml of media and 12 g ml 1 polybrene (Santa Cruz Biotechnology, SC-134220), and then added to one well of a six-well plate containing MEFs that were plated 24 h before at around 15,600 cells per cm 2 , for a final 8 g ml 1 polybrene

This illustrates that human gut microbiota can detect and react to changes in luminal metabolites resulting from fiber fermentation, connecting microbial metabolism with host hormone control

It neutralizes free radicals , which are unstable molecules that can cause cellular damage if left unchecked