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After TRAP staining, the cells were washed three times with PBS and stained with Actistain 488 Fluorescent Phalloidin (Cytoskeleton, Denver, CO, United States) at room temperature for 30 min in the dark

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This can explain why ifenprodil has been approved for clinical use in Japan and France, and a similar compound in the USA (107)

Identification, selection and immune assessment of liver stage CD8 T cell epitopes from plasmodium falciparum