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We summarize the main environmental and ecological factors that can influence the host-parasite interaction in polluted environments (Figure 2), including (i) the type of pollutants mixture, their probabilities of occurrence, and their chemical properties (accumulation, metabolization, differential toxicity among species, etc.), (ii) the nature and intensity of parasites, tolerance to pollutants, the ability to sequester pollutants, life cycle (number of obligatory hosts), diversity of parasites species, and dynamics of co-infection, and (iii) host tolerance and resistance to parasites, tolerance to pollutants, individual traits (sex, age, body length, trophic ecology, etc.), and inter- and intra-specific relationships

After 30 minutes of antibiotic treatment at 37C, the cells were washed three times with PBS

These results suggest that microbial exposure is not the only trigger of disease development

In brief, samples were analyzed on a "PrinCe 706 CE system equipped with an uncoated capillary (85 cm 50 m ID) and a laboratory-constructed CE-ICP-MS interface for element selective quantification of separated iron redox species at ICP-DRC (dynamic reaction cell)-MS