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Endocrinology Reviews, 39(2), 333350

doi: 10.1007/s11528-022-00759-0 337

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Pull down assay for GPX4 PC12 cells were treated with DAQ probe (DA-yne probe contains FeCl 3 solution) or DMSO for 6 hours, and then were harvested and lysed with RIPA buffer supplemented with protease inhibitor cocktails and phosphatase inhibitor cocktails