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The peptides were separated and eluted with a gradient of 1080% buffer B (0.1% formic acid, 90% acetonitrile/in water) in buffer A (0.1% formic acid, 2% acetonitrile/in water) at a flow rate of 500 nl/min over 60 min

PLoS Biol (2010) 8(6):e1000412.10.1371/journal.pbio.1000412 42 HoSNHuntHDHortonRMPullenJKPeaseLR

Intriguingly, under conditions of caspase-1 deficiency or pharmacological inhibition, an alternative pathway emerges: the inflammasome redirects its signaling to activate caspase-3, which subsequently processes GSDME to execute pyroptosis [80]

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