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Given the high prevalence of clinical and subclinical B 12 deficiency in the elderly, the role of B 12 in myelination, and B 12 deficiency as a differential diagnosis of DCM, there is considerable rationale to conduct routine assessment of B 12 levels in patients with DCM

But research consistently shows that neurological and metabolic dysfunction begins at levels below 400500 pg/mL in many individuals

Cells were then fixed with 4% paraformaldehyde (PFA) for 10 min at room temperature, followed by permeabilization with 0.25% Triton X-100 (Servicebio, China) in PBS for 10 min

For individuals, following a plant-based diet, fortified plant-based milk, and nutritional yeast can help bridge the gap