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No adverse effects were reported and a significant reduction in liver tests (specifically transaminases and gamma-glutamyltranspeptidase -GGT-), with many cases of bio humoral parameters normalization, was detected in all treated patients, even several months after treatment interruption

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As the freezing of plant tissue material consisted of small amounts of cut plant material pieces, we conclude that the freezing of tissue led to faster freezing rates and to denaturation of enzymes, while the comparably bigger amounts of extracts had slower freezing rates