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PMID: 38839992 L-Thyroxine purchased from MedChemExpress

95 % CI (039, 010)

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For immunogold labelling high-pressure frozen worms were rapidly freeze substituted with the help of a Leica AFS fitted with an agitation module 57 in 0.2% uranyl acetate, 5% H 2 O in acetone as follows: from 110 C up to 50 C over 2 hours, pause at 50 C, 2 5 min washes with acetone, 2 5 min washes with ethanol, successive 20%, 40%, 60%, 80%, and 100% Lowicryl (Electron Microscopy Sciences, Hatfield, PA) over 2 hours and overnight incubation in 100% Lowicryl