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2.8 Detection of reactive oxygen species level The level of ROS in HGC27 cells and AGS cells was examined using a ROS assay kit with CM-H2DCFDA (S0035S, Beyotime) in line with the manufacturers manual

The analysis was performed separately for upregulated and downregulated proteins with a fold change greater than 1.5 against biological processes of Gene Ontology (Ashburner et al