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In mice plasma TMAO has been shown to be higher in female and TMA lower compared to males as Flavin-Containing Monooxygenase 3 (the main TMAO producing enzyme) expression is drastically downregulated by testosterone (and mildly upregulated by estrogen

However, recently, BA has been associated, directly or indirectly, with immune signaling, metabolism, differentiation, and microbiota modulation (90, 91)