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Mechanistic evidence, as well as a scattering of ecological and cross-sectional studies, suggests that vitamin D may play an important role in the aetiology of ASD

After that, the cells were stained in 1 mM FerroOrange (Dojindo, Kumamoto, Japan) in HBSS for exactly 30 min at 37 C and 5% CO2 and imaged immediately with a fluorescence microscope