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Concurrently, activation of the IRE1-JNK pathway phosphorylates the pro-apoptotic protein Bim and inhibits the anti-apoptotic function of Bcl-2, ultimately activating Caspase-3 to execute the cell death program [356]

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After fixation and washing, sections were immersed in fresh -gal staining solution and incubated at 37 C overnight

Changes in ionic strength induced by high salt solutions are a second way for nonspecific elution